Michael and the team at the Wisconsin Crop Innovation Center have invented a genotype
independent transformation protocol that has successfully created over 500 independent engineered or edited cannabis lines. Several traits have been targeted for modification and results are shared in the poster.

Highlights
• Proven transformation method generating stable and heritable progeny
• Generated the first gene edited hemp line to be granted USDA non-regulated status. This line, called Badger G, is a THC and CBD-free line that is high in CBGA and was created by knocking out the CBDAS enzyme through gene editing
• Created a totally cannabinoid-free line, Badger Zero, by gene editing the TKS gene
• Generated a powdery mildew resistant variety, Badger PMR, by gene editing key MLO sensitive genes
• Overexpressed some transcription factors that increased anthocyanin and flavonoid production
• Modified terpene profiles by overexpressing certain terpene synthase genes

We hope this work helps to bring these new breeding technologies into the cannabis realm and increase our knowledge of a fascinating species!